Hippocampus
How many neurons -- thousands
20 minutes -- 60 per second. Calcium numbers per second..

60 * 60 * 20 * 1000 = 72 million

Classify what is sub-threshold.
Correlate different neurons. Could be delayed correlation.
Ensembles can be detected using calcium signals.
ICA analysis.

Groups of cells. Image them every day.
Change in calcium signal. Do we see increase in particular events.
Does it occur in the same time in the behavior.
Do the set of cells that are firing change.
Are the highly active cells that are always recruited.
Low activity ones show more modularity.

delta F/Flourescence
Basal flourescence.

For spectral: get peaks and integrals. Onset value.


Compare high amounts of activity with
low amounts of activity.

Dear Jayeeta,

Here is what I would need:
definition of onset,
definition of activity

Data that somehow identifies neuron, location.
Then neuron, time, calcium flourescence.
So two files, pure text.

Thanks,
Dennis
